Human Carboxypeptidase A2/CPA2 Antibody

Catalog #: MAB28961 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB28961
MAB28961-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase A2/CPA2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA4, rhCPB1, or rhCPE is observed. Recognizes the pro form (aa 17‑417), but not the activated enzyme (aa 113‑417) in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 384023

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Leu18-His417
Accession # P48052

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carboxypeptidase A2/CPA2 (Catalog # 2896-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/
CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (aa 1 to 16), a pro region (aa 17 to 112), and a mature chain (aa 113 to 417). The amino acid sequence of human CPA2 is 99.5%, 90%, 87%, 86% and 70% identical to that of chimpanzee, canine, rat, mouse and chicken.

References

Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Entrez Gene IDs

1358 (Human)

Alternate Names

carboxypeptidase A2 (pancreatic); Carboxypeptidase A2; CPA2; EC 3.4.17; EC 3.4.17.15

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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