Home / Carboxypeptidase B1/CPB1 / Human Carboxypeptidase B1/CPB1 Antibody

Human Carboxypeptidase B1/CPB1 Antibody

Catalog #: MAB2897
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2897
MAB2897-SP
Product Details
Citations (2)
FAQs
Supplemental Products
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase B1/CPB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPE, recombinant mouse (rm) CPA4, and rmCPB1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 438806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B1/CPB1
His16-Tyr417
Accession # P15086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis (2). The deduced amino acid (aa) sequence of human CPB1 consists of a signal peptide (aa 1-15), a pro region (aa 16-110), and a mature chain (aa 111-417). Human CPB1 shares 98%, 84%, 76%, 75%, and 84% aa sequence identity with chimpanzee, dog, rat, mouse and chicken CPB1, respectively.

References
  1. Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 831, Academic Press, San Diego.
  2. Yamamoto, K.K. et al. (1992) J. Biol. Chem. 267:2575.
Entrez Gene IDs
1360 (Human); 76703 (Mouse)
Alternate Names
carboxypeptidase B; carboxypeptidase B1 (tissue); Carboxypeptidase B1; CPB; CPB1; DKFZp779K1333; EC 3.4.17; EC 3.4.17.2; Pancreas-specific protein; PASP; PCPB; procarboxypeptidase B

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Citations for Human Carboxypeptidase B1/CPB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models
    Authors: J Beumer, J Bauzá-Mart, TS Veth, V Geurts, C Boot, H Gilliam-Vi, SS Poulsen, FK Knop, W Wu, H Clevers
    Proceedings of the National Academy of Sciences of the United States of America, 2022-11-07;119(46):e2212057119.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  2. Fecal host biomarkers predicting severity of Clostridioides difficile infection
    Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
    JCI Insight, 2021-01-11;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

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