Human Carboxypeptidase B2/CPB2 Antibody

Catalog #: AF6036 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6036
AF6036-SP

Detection of Human Carboxypeptidase B2/CPB2 by Western Blot.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase B2/CPB2 Antibody Summary

Species Reactivity

Human

Specificity

Detects Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CPA1, rhCPB1 and recombinant mouse CPB1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2
Phe23-Val423 (Ala169Thr)
Accession # Q96IY4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human platelets. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase B2/CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxypeptidase B2/CPB2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

View Larger

Carboxypeptidase B2/CPB2 in Human Liver and Liver Cancer Tissue. Carboxypeptidase B2/CPB2 was detected in immersion fixed paraffin-embedded sections of normal human liver (left panel) and human liver cancer tissue (right panel) using Sheep Anti-Human Carboxypeptidase B2/ CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase B2/CPB2

CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes. It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation. CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa). Active CPB2 removes C-terminal Lys residues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization. Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length. It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminal Lys or Arg residues. There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423. Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.

Entrez Gene IDs

1361 (Human); 56373 (Mouse); 113936 (Rat)

Alternate Names

carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); Carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 3.4.17.20; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor