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Human Carboxypeptidase E/CPE Antibody

Catalog #: MAB3587
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3587
MAB3587-SP
Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase E/CPE Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPB1, or recombinant mouse CPA4 is observed.
Source
Monoclonal Mouse IgG2A Clone # 420709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase E/CPE
Arg42-Ser453
Accession # P16870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase E/CPE

Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters. CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance. The CPE precursor includes a signal peptide (aa 1‑25), a propeptide (aa 26‑42) and a mature chain (aa 43‑476). The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken.

Entrez Gene IDs
1363 (Human); 12876 (Mouse); 25669 (Rat)
Alternate Names
Carboxypeptidase E; Carboxypeptidase H; CPE; CPH; CPH-1; EC 3.4.17; EC 3.4.17.10; Enkephalin convertase; Prohormone-processing carboxypeptidase

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Citations for Human Carboxypeptidase E/CPE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A Novel Single Nucleotide T980C Polymorphism in the Human Carboxypeptidase E Gene Results in Loss of Neuroprotective Function
    Authors: L Cong, Y Cheng, NX Cawley, SR Murthy, YP Loh
    PLoS ONE, 2017-01-23;12(1):e0170169.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A human carboxypeptidase E/NF-?1 gene mutation in an Alzheimer's disease patient leads to dementia and depression in mice
    Authors: Y Cheng
    Transl Psychiatry, 2016-12-06;6(12):e973.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers.
    Authors: Lee TK, Murthy SR, Cawley NX, Dhanvantari S, Hewitt SM, Lou H, Lau T, Ma S, Huynh T, Wesley RA, Ng IO, Pacak K, Poon RT, Loh YP
    J. Clin. Invest., 2011-03-01;121(3):880-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. NOS1AP regulates dendrite patterning of hippocampal neurons through a carboxypeptidase E-mediated pathway.
    Authors: Carrel D, Du Y, Komlos D, Hadzimichalis NM, Kwon M, Wang B, Brzustowicz LM, Firestein BL
    J. Neurosci., 2009-06-24;29(25):8248-58.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation

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