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Human Carboxypeptidase E/CPE Antibody

Catalog #: AF3587
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3587
AF3587-SP
Carboxypeptidase E/CPE in HepG2 Human Cell Line.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase E/CPE Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human CPM is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase E/CPE
Arg42-Ser453
Accession # P16870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Carboxypeptidase E/CPE antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Carboxypeptidase E/CPE in HepG2 Human Cell Line. Carboxypeptidase E/CPE was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Carboxypeptidase E/CPE antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Carboxypeptidase E/CPE in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol and saponin.

Immunohistochemistry Carboxypeptidase E/CPE antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Carboxypeptidase E/CPE in Mouse Embryo. Carboxypeptidase E/CPE was detected in immersion fixed frozen sections of mouse embryo (9.5 d.p.c.) using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing liver. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase E/CPE

Encoded by the CPE gene and also known as Carboxypeptidase H, CPE is a single chain peptidase with an optimal pH range between 5.0‑6.0. It is a zinc metallocarboxypeptidase that removes basic amino acids from the C-terminus of peptides (1). Like other metallocarboxypeptidases, its activity is stimulated by millimolar concentrations of Co2+. Its activity is regulated by pH-induced aggregation above pH 6.0. Its major function seems to process numerous peptide hormones and neurotransmitters. In addition to its proteolytic function, it also plays a role as a sorting receptor (2), which may be attributed to the sorting of this protein into the secretory pathway. The C-terminal domain of CPE causes the peripheral association of CPE with membranes below neutral pH, resulting in the association of this protein into membranes (3). CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance (4).

References
  1. Fricker, L.D. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 840.
  2. Cool, D.R. et al. (1997) Cell 88:73.
  3. Zhang, C-F. et al.  (2003) Biochem. J. 369:453.
  4. Cawley, N.X. et al. (2004) Endocrinology 145:5807.
Entrez Gene IDs
1363 (Human); 12876 (Mouse); 25669 (Rat)
Alternate Names
Carboxypeptidase E; Carboxypeptidase H; CPE; CPH; CPH-1; EC 3.4.17; EC 3.4.17.10; Enkephalin convertase; Prohormone-processing carboxypeptidase

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Citations for Human Carboxypeptidase E/CPE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  2. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  3. Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production
    Authors: H Chen, AK Victor, J Klein, KF Tacer, DJ Tai, C de Esch, A Nuttle, J Temirov, LC Burnett, M Rosenbaum, Y Zhang, L Ding, JJ Moresco, JK Diedrich, JR Yates, HS Tillman, RL Leibel, ME Talkowski, DD Billadeau, LT Reiter, PR Potts
    JCI Insight, 2020-09-03;5(17):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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