Human CARD9 Antibody

Catalog #: AF5248 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5248
AF5248-SP

Detection of Human CARD9 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CARD9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CARD9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CARD6, rhCARD8, rhCARD11, rhCARD14, and rhCARD10 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CARD9
Lys225-Gln359
Accession # Q9H257

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CARD9 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CARD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CARD9 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CARD9

CARD9 (caspase recruitment domain-containing protein 9) is a 70 kDa member of the CARD module family of proteins. It is expressed in myeloid lineage cells such as macrophages, neutrophils and dendritic cells and mediates activation of p38 and Jnk, as well as NF-kappa B by interacting with BCL10 and MALT1 in a PKC-independent manner. Human CARD9 is 536 amino acids (aa) in length. It contains one CARD domain (aa 6‑98), two coiled-coil regions (aa 117‑277 and 332‑419) and a phosphorylation site at Ser460. CARD9 dimerizes with itself and BCL10. Multiple potential splice variants are noted for CARD9. There are 35 aa and 39 aa substitutions for aa 479‑536, a seven aa substitution for aa 360‑536 and an eleven aa substitution for aa 482‑536. Over aa 9‑256, human CARD9 is 88% aa identical to mouse CARD9.

Long Name

Caspase Recruitment Domain Containing Protein 9

Entrez Gene IDs

64170 (Human); 332579 (Mouse); 64171 (Rat)

Alternate Names

CANDF2; CARD9; caspase recruitment domain family, member 9; caspase recruitment domain-containing protein 9; hCARD9