Human Cardiotrophin-1/CT-1 Antibody Summary
Ser2-Ala201
Accession # Q16619
Applications
This antibody functions as an ELISA captureantibody when paired with Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602).
This product is intended for assay development onvarious assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human Cardiotrophin-1/CT-1 ELISA Standard Curve. Recombinant human Cardiotrophin/CT-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cardiotrophin-1/CT-1
Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.
Product Datasheets
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