Human Carnosine Dipeptidase 1/CNDP1 Antibody

Catalog #: MAB24891 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24891
MAB24891-SP

All Carnosine Dipeptidase 1/CNDP1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carnosine Dipeptidase 1/CNDP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 352213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
Pro28-His507
Accession # Q96KN2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Carnosine Dipeptidase 1/CNDP1 (0.5 µg/mL, Catalog # 2489-ZN) cleavage of the fluorogenic peptide substrate L-Carnosine (0.5 mM). The Neutralization Dose (ND₅₀) is typically 0.6 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carnosine Dipeptidase 1/CNDP1

The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase. CNDP1 consists of a signal peptide (residues 1 to 26) and the mature chain (residues 27 to 507). The amino acid sequence of human CNDP1 is 97%, 85%, 82% and 67% identical to that of chimpanzee, canine, mouse/rat and chicken.

References

Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.

Entrez Gene IDs

84735 (Human)

Alternate Names

beta-Ala-His dipeptidase; Carnosinase 1; carnosine dipeptidase 1 (metallopeptidase M20 family); Carnosine Dipeptidase 1; CN1MGC102737; CNDP dipeptidase 1; CNDP1; CPGL2; CPGL2MGC142072; EC 3.4.13.20; Glutamate carboxypeptidase-like protein 2; HsT2308; MGC10825; Serum carnosinase