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Human CART aa 28-116 Antibody

Catalog #: MAB163
11 Citations Datasheet / COA / SDS
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MAB163
MAB163-SP
Product Details
Citations (11)
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Human CART aa 28-116 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CART aa 28‑116 in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.
Source
Monoclonal Mouse IgG2B Clone # 113612
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CART
Ala37-Leu116
Accession # Q16568
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CART
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of rat brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

CART, also known as cocaine and amphetamine-related transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations.  In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment.  There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region.  There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist.  These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89).  Human CART is 98% aa identical to both moyuse and rat CART (1-89).  Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions.   In addition, anatomical location is associated with differing lengths of CART.  For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References
  1. Douglass, J. & S. Daoud (1996) Gene 169:241.
  2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
  3. Adams, L.D. et al. (1999) Brain Res. 848:137.
  4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
  5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
  6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
  7. Thim, L. et al. (1998) FEBS Lett. 428:263.
Long Name
Cocaine- and Amphetamine-regulated Transcript Protein
Entrez Gene IDs
9607 (Human)
Alternate Names
CART prepropeptide; CART; CARTcocaine and amphetamine regulated transcript; CARTPT; cocaine- and amphetamine-regulated transcript protein

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Citations for Human CART aa 28-116 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Distribution and Chemistry of Phoenixin-14, a Newly Discovered Sensory Transmission Molecule in Porcine Afferent Neurons
    Authors: Mazur, U;Lepiarczyk, E;Janikiewicz, P;?opie?ska-Biernat, E;Majewski, MK;Bossowska, A;
    International journal of molecular sciences
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
    Authors: Kobori, N;Moore, AN;Redell, JB;Dash, PK;
    Journal of neuroinflammation
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
    Authors: Kobori, N;Moore, AN;Redell, JB;Dash, PK;
    Journal of neuroinflammation
    Species:  Human
    Sample Types: Protein
  4. Astrocyte heterogeneity within white matter tracts and a unique subpopulation of optic nerve head astrocytes
    Authors: Arpan G. Mazumder, Amélie M. Julé, Paul F. Cullen, Daniel Sun
    iScience
  5. The Influence of Bisphenol a on the Nitrergic Nervous Structures in the Domestic Porcine Uterus
    Authors: L Rytel, S Gonkowski
    Int J Mol Sci, 2020-06-26;21(12):.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  6. Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach
    Authors: K Palus, M Bulc, M Czajkowska, B Mici?ski, J Ca?ka
    Tissue Cell, 2017-12-05;50(0):8-14.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  7. Cocaine- and amphetamine-regulated transcript peptide and calcium binding proteins immunoreactivity in the superficial layers of the superior colliculus in the guinea pig: Implications for visual sensory processing
    Authors: Janusz Najdzion
    J. Chem. Neuroanat, 2016-11-28;79(0):66-79.
    Species: Guinea Pig
    Sample Types: Whole Tissue
    Applications: IHC
  8. Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica)
    Authors: Malgorzata Radzimirska, Krystyna Bogus-Nowakowska, Tadeusz Kuder, Anna Robak
    Folia Histochemica et Cytobiologica
  9. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
    Authors: Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T
    PLoS ONE, 2014-12-31;9(12):e115731.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC-Fr
  10. Expression of the ghrelin receptor gene in neurons of the medulla oblongata of the rat
    Authors: Romke Bron, Lei Yin, Domenico Russo, John B. Furness
    Journal of Comparative Neurology
  11. Chemical coding for cardiovascular sympathetic preganglionic neurons in rats.
    Authors: Gonsalvez DG, Kerman IA, McAllen RM
    J. Neurosci., 2010-09-01;30(35):11781-91.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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