Human Casein Kinase 2 alpha Antibody

Detection of Human, Mouse, and Rat Casein Kinase 2 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Casein Kinase 2a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Casein Kinase 2 alpha in HEK293 Human Cell Line. Casein Kinase 2a was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human and Mouse Casein Kinase 2 alpha by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.5 mg/mL. A specific band was detected for Casein Kinase 2 alpha at approximately 50-52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Casein Kinase 2 alpha in Human Brain. Casein Kinase 2a was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human Casein Kinase 2 alpha Specificity Using Knockout Cell Line. Western blot shows lysates of HAP1 human colorectal carcinoma cell line and Casein Kinase 2 alpha knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated goat anti-mouse IgG Secondary Antibody. A specific band was detected for Casein Kinase 2 alpha at approximately 47 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Casein Kinase 2 alpha Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HAP1 WT and Casein Kinase 2 alpha KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) followed by incubation with a goat anti-mouse Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).