Human Caspase-1 Antibody

Detection of Human Caspase‑1 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caspase‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Caspase‑1 in U937 Human Cell Line. Caspase‑1 was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive) and MCF‑7 human breast cancer cell line (negative control) using Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human Caspase‑1 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caspase‑1 at approximately 52 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human Caspase‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase‑1 knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caspase‑1 at approximately 48 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human Caspase‑1 ELISA Standard Curve. Recombinant Human Caspase‑1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Caspase‑1 Monoclonal Antibody (MAB62151) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).