Human Caspr1 Antibody

Catalog #: MAB7548 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7548
MAB7548-SP

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Human Caspr1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspr1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Caspr2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 753232

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Caspr1
Trp20-Gly1280
Accession # P78357

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Caspr1 in IMR32 Human Cell Line. Caspr1 was detected in immersion fixed IMR32 human neuroblastoma cell line using Mouse Anti-Human Caspr1 Monoclonal Antibody (Catalog # MAB7548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Detection of Caspr1 in A172 human glioblastoma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative). Caspr1 was detected in immersion fixed A172 human glioblastoma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative) using Mouse Anti-Human Caspr1 Monoclonal Antibody (Catalog # MAB7548) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr1

Contactin-associated protein 1 (Caspr1), also known as Neurexin-4, Paranodin, and NCP1, is an approximately 190 kDa transmembrane protein that is concentrated at paranodal junctions of axons during myelination. It is required for junction formation and ion channel localization and plays an important role in the propagation of saltatory nerve conduction. Caspr1 interacts with Contactins and Nogo-A at the paranodal junction and inhibits the binding of Contactin to glial cell Neurofascin. Caspr1 is proteolytically cleaved by Reelin, and this action is inhibited by the cellular prion protein (PrP). Mature human Caspr1 consists of a 1264 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 80 aa cytoplasmic domain. The ECD contains one Factor 5/8-like domain, four Laminin-G-like domains, two EGF-like domains, and one Fibrinogen-like domain. Within the ECD, human Caspr1 shares 95% aa sequence identity with mouse and rat Caspr1.

Long Name

Contactin Associated Protein-like 1

Entrez Gene IDs

8506 (Human); 53321 (Mouse)

Alternate Names

Caspr; Caspr1; CASPRcaspr1; CNTNAP; CNTNAP1; contactin associated protein 1; neurexin 4; Neurexin IV; neurexin-4; NRXN4contactin-associated protein 1; p190