Human Cathepsin B Biotinylated Antibody

Catalog #: BAF953
1 Citation Datasheet / COA / SDS

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BAF953

All Cathepsin B Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin B Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cathepsin B in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Cathepsin A, C, D, E, L, S, V, X/Z/P, rhCystatin C, D, SA, recombinant mouse (rm) Cystatin C, D, SA, and rmCathepsin B is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin B
Arg18-Ile339
Accession # P07858

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cathepsin B (Catalog # 953-CY)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human normal brain and bladder cancer tissue

Human Cathepsin B Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human Pro‑Cathepsin B Antibody (Catalog # MAB2176)

Standard: Recombinant Human Cathepsin B Protein, CF (Catalog # 953-CY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin B

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin, and secretory leucoprotease inhibitor (SLPI) (2-4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution of the enzyme in human and animal tumors, Cathepsin B may also have role in invasion and metastasis (5).

In addition to lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (1).

References

Mort, J.S. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): Academic Press, San Diego, p. 1079.
Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
Jutras, I. and T.L. Reudelhuber (1999) FEBS Lett. 443:48.
Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
Bergquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.

Entrez Gene IDs

1508 (Human); 13030 (Mouse)

Alternate Names

APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Cathepsin B Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1