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Human Cathepsin E Antibody

Catalog #: MAB1294 1 Review Datasheet / COA / SDS
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MAB1294
MAB1294-SP
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Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin E Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and mature forms of recombinant human (rh) Cathepsin E. In Western blots, 100% cross‑reactivity with recombinant mouse (rm) Cathepsin E and recombinant human (rh) BACE is observed under reducing conditions. Under non-reducing conditions, less than 1% cross-reactivity with rmCathepsin E and rhBACE-1 is observed. Less than 1% cross-reactivity with rhCathepsin D is observed under both reducing and non-reducing conditions.
Source
Monoclonal Mouse IgG2A Clone # 212211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin E
Gln18-Pro396
Accession # NP_001901
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cathepsin E (Catalog # 1294-AS)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin E (Catalog # 1294-AS), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta  site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (aa 1‑17), a propeptide (aa 18‑53), and a mature chain (aa 54‑396) (4).

References
  1. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
  2. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  3. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
  4. Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.
Entrez Gene IDs
1510 (Human); 13034 (Mouse)
Alternate Names
CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase

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Human Cathepsin E Antibody
By Sam Ivry on 04/21/2016
Application: WB Sample Tested: Adult pancreas,Recombinant protein Species: Human

1:500 dilution in 5% milk (PBS with Tween)
Lane 1: Ladder
Lane 2: Recombinant CTSE
Lane 3: Activated recombinant CTSE
Lane 4: Adult pancreas

Western Blot Human Cathepsin E Antibody MAB1294