Home / Cathepsin V / Human Cathepsin V Antibody

Human Cathepsin V Antibody

Catalog #: AF1080
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1080
AF1080-SP
Cathepsin V in Human Breast Cancer Tissue.
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Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin V Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin V in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Cathepsin C, rhCathepsin S, rhCathepsin H, rhCathepsin F and rhCathepsin L is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin V
Val18-Val334
Accession # O60911
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin V (Catalog # 1080-CY)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Human Cathepsin V (0.5 µg/mL, Catalog # 1080-CY) cleavage of the fluorogenic peptide substrate Z-LR-AMC (10 µM, Catalog # ES008). The Neutralization Dose (ND50) is typically 2.2 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Cathepsin V antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cathepsin V in Human Breast Cancer Tissue. Cathepsin V was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human Cathepsin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin V

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

References
  1. Turk, V. et al. (2001) EMBO J. 20:4629.
  2. Bromme, D. et al. (1999) Biochemistry 38:2377.
  3. Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Entrez Gene IDs
1515 (Human)
Alternate Names
Cathepsin L2; Cathepsin U; Cathepsin V; CATL2; CTSL2; CTSUcathepsin L2, preproprotein; CTSVMGC125957; EC 3.4.22; EC 3.4.22.43

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Citations for Human Cathepsin V Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer
    Authors: Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden
    Breast Cancer Research
  2. The abnormal accumulation of heparan sulfate in patients with mucopolysaccharidosis prevents the elastolytic activity of cathepsin V
    Authors: T Chazeirat, S Denamur, KK Bojarski, PM Andrault, D Sizaret, F Zhang, A Saidi, M Tardieu, RJ Linhardt, F Labarthe, D Brömme, SA Samsonov, G Lalmanach, F Lecaille
    Carbohydrate polymers, 2020-10-20;253(0):117261.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Deficiency for the cysteine protease cathepsin L promotes tumor progression in mouse epidermis.
    Authors: Dennemarker J, Lohmuller T, Mayerle J, Tacke M, Lerch MM, Coussens LM, Peters C, Reinheckel T
    Oncogene, 2009-12-21;29(11):1611-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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