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Human Cathepsin X/Z/P Biotinylated Antibody

Catalog #: BAF934 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF934
Cathepsin X/Z/P in Human Breast Cancer Tissue.
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Human Cathepsin X/Z/P Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepzin X/Z/P in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, and rhCathepsin L is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY)
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Cathepsin X/Z/P in Human Breast Cancer Tissue. Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Cathepsin X/Z/P Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF934) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References
  1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
  2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
  3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
  4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
  5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
  6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, R. et al. (2001) Biol. Chem. 382:839.
  8. Therrien, C. et al. (2001) Biochemistry 40:2702.
  9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
Entrez Gene IDs
1522 (Human); 64138 (Mouse)
Alternate Names
Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSX; CTSZ

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