Human CCK Antibody

CCK in Capan‑2 Human Cell Line. CCK was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CCK in Human Duodenum. CCK was detected in immersion fixed paraffin-embedded sections of human duodenum using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuroendocrine cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.