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Human CCL1/I-309/TCA-3 Antibody

Catalog #: MAB272
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB272-SP
MAB272-500
MAB272-100
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti-body.
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Citations (4)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma, 11, 21, or 25 is observed.
Source
Monoclonal Mouse IgG1 Clone # 35305
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL1/I‑309/TCA‑3
Lys24-Lys96
Accession # P22362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)

Human CCL1/I-309 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF272)

Standard: Recombinant Human CCL1/I-309 Protein (Catalog # 272-I)

Neutralization
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti-body. View Larger

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti-body. Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I-309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N‑linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References
  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Citations for Human CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD34+CD10+CD19- Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization
    Authors: A Asai, Y Tsuchimoto, H Ohama, H Nishikawa, A Chopra, K Higuchi
    Cells, 2022-08-30;11(17):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC
    Authors: A Asai, Y Tsuchimoto, H Ohama, S Fukunishi, Y Tsuda, M Kobayashi, K Higuchi, F Suzuki
    Oncoimmunology, 2017-03-03;6(4):e1299301.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. CC chemokine ligand 1 is released into the airways of atopic asthmatics.
    Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
    Eur. Respir. J., 2006-03-15;28(1):59-67.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development, Neutralization
  4. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001-10-01;70(4):617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Human CCL1/I-309/TCA-3 Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.

ELISA Human CCL1/I-309/TCA-3 Antibody MAB272