Home / CCL13/MCP-4 / Human CCL13/MCP‑4 Biotinylated Antibody

Human CCL13/MCP‑4 Biotinylated Antibody

Catalog #: BAF327
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF327
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL13/MCP‑4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL13/MCP-4 in ELISAs and Western blots. In sandwich immunoassays, approximately 0.3% cross-reactivity with recombinant mouse (rm) JE, recombinant human (rh) MCP-2, rhMCP-3, rmMARC, rmMCP-5, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES, and rmRANTES is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL13/MCP-4 (R&D Systems, Catalog # 327-P4)
Gln24-Thr98
Accession # Q99616
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL13/MCP‑4 (Catalog # 327-P4)

Human CCL13/MCP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL13/MCP‑4 Antibody (Catalog # MAB327)

Standard: Recombinant Human CCL13/MCP-4 Protein (Catalog # 327-P4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL13/MCP-4

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, -3, and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR2 and CCR3, both of which have been shown to bind CCL13.

References
  1. Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
  2. Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
  3. Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
  4. Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
  5. Heath, H. et al. (1997) J. Clin. Invest. 99:178.
  6. Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
  7. Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
  8. Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.
Entrez Gene IDs
6357 (Human)
Alternate Names
C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human CCL13/MCP‑4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  2. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CCL13/MCP‑4 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human CCL13/MCP‑4 Biotinylated Antibody and earn rewards!

Have you used Human CCL13/MCP‑4 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review