Human CCL17/TARC Antibody

Catalog #: AF364
13 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF364
AF364-SP

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.

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All CCL17/TARC Antibodies

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Citations (13)

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Human CCL17/TARC Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
Ala24-Ser94
Accession # Q92583

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CCL17/TARC (Catalog # 364-DN)

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL17/TARC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/ TARC (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND₅₀ is typically 0.3-1.5 µg/mL.

Immunohistochemistry

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CCL17/TARC in Human Spleen. CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

References

Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
Nomiyama, H. et al. (1997) Genomics 40:211.

Entrez Gene IDs

6361 (Human); 20295 (Mouse)

Alternate Names

ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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Related Research Areas

Citations for Human CCL17/TARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Prognostic value of TARC and quantitative PET parameters in relapsed or refractory Hodgkin lymphoma patients treated with brentuximab vedotin and DHAP
Authors: J Driessen, MJ Kersten, L Visser, A van den Be, SH Tonino, JM Zijlstra, PJ Lugtenburg, F Morschhaus, M Hutchings, S Amorim, T Gastinne, M Nijland, GJC Zwezerijne, R Boellaard, HCW de Vet, AIJ Arens, R Valkema, RDK Liu, EEE Drees, D de Jong, WJ Plattel, A Diepstra, HOVON Lune
Leukemia, 2022-10-14;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
C-C Motif Chemokine Ligand-17 as a Novel Biomarker and Regulator of Epithelial Mesenchymal Transition in Renal Fibrogenesis
Authors: YH Hsieh, WC Wang, TW Hung, CC Lee, JP Tsai
Cells, 2021-11-29;10(12):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Authors: Rump L, Mattey D, Kehoe O, Middleton J
Cytokine, 2017-09-01;97(0):133-140.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis
Authors: Y Yuan, J Kluiver, J Koerts, D de Jong, B Rutgers, FRA Razak, M Terpstra, B Plaat, IM Nolte, A Diepstra, L Visser, K Kok, A van den Be
Am. J. Pathol., 2017-04-20;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010-02-17;184(6):2886-98.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
Authors: Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
Thorax, 2008-02-14;63(9):810-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
Blood, 2007-12-10;111(4):2339-46.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
J. Immunol., 2007-06-15;178(12):7678-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
Authors: Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, Van Den Berg A
J. Pathol., 2006-08-01;209(4):474-83.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
TARC augments TNF-alpha-induced CTACK production in keratinocytes.
Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
Exp. Dermatol., 2004-09-01;13(9):551-7.
Species: Human
Sample Types: Cell Culture Supernates

Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
Int. J. Oncol., 2004-09-01;25(3):605-13.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
Int. J. Cancer, 2002-06-10;99(5):665-72.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC

Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer
Authors: Michael Kvorjak, Yasmine Ahmed, Michelle L. Miller, Raahul Sriram, Claudia Coronnello, Jana G. Hashash et al.
Cancer Immunology Research