Home / CCL18/PARC / Human CCL18/PARC Fluorescein-conjugated Antibody

Human CCL18/PARC Fluorescein-conjugated Antibody

Catalog #: IC394F
1 Citation Datasheet / COA / SDS

Discontinued Product

IC394F has been discontinued.
View all CCL18/PARC products.
Detection of CCL18/PARC in Human Monocyte-derived Dendritic Cells by Flow Cytometry.
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Human CCL18/PARC Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL18/PARC in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL3, 4, 14, 15, 23, recombinant mouse (rm) CCL3, rmCCL4, recombinant viral (rv) MIP-I or rvMIP‑II.
Source
Monoclonal Mouse IgG1 Clone # 64507
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CCL18/PARC antibody in Human Monocyte-derived Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of CCL18/PARC in Human Monocyte-derived Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL18/PARC Fluorescein-conjugated Monoclonal Antibody (Catalog # IC394F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL18/PARC

CCL18, also known as Alternative Macrophage Activation-associated CC Chemokine 1 (AMAC-1), Macrophage Inflammatory Protein 4 (MIP-4), and Dendritic Cell Chemokine 1 (DC‑CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN-gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.

Entrez Gene IDs
6362 (Human)
Alternate Names
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

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Citation for Human CCL18/PARC Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mature dendritic cells express functional thrombin receptors triggering chemotaxis and CCL18/pulmonary and activation-regulated chemokine induction.
    Authors: Li X, Syrovets T, Paskas S, Laumonnier Y, Simmet T
    J. Immunol., 2008-07-15;181(2):1215-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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