Human CCL19/MIP-3 beta QuicKit ELISA New

Catalog #: QK0361 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
QK0361
SK0361
PK0361

Control Products Available

QC318: QuicKit Control for Human CCL19/MIP-3 beta

Human CCL19/MIP-3 beta Quantikine QuicKit ELISA Standard Curve

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All CCL19/MIP-3 beta ELISAs

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CCL19/MIP-3 beta QuicKit ELISA Summary

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)

Sensitivity

0.695 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MIP-3 beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine QuicKit Human CCL19/MIP-3 beta Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human MIP-3 beta levels in cell culture supernates, serum, and plasma. It contains recombinant human MIP-3 beta and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 beta showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural MIP-3 beta .

Recovery

The recovery of human MIP-3 beta spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	112	106-117
EDTA Plasma (n=2)	99	91-107
Heparin Plasma (n=2)	98	93-102
Serum (n=2)	98	94-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human MIP-3 beta were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data

N/A

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Human CCL19/MIP-3 beta Quantikine QuicKit ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs:

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names:

beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19