Human CCL19/MIP-3 beta QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MIP-3 beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine QuicKit Human CCL19/MIP-3 beta Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human MIP-3 beta levels in cell culture supernates, serum, and plasma. It contains recombinant human MIP-3 beta and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 beta showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural MIP-3 beta .
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
1 |
2 |
| n |
20 |
20 |
10 |
10 |
| Mean (pg/mL) |
42.4 |
326 |
45.8 |
338 |
| Standard Deviation |
3.15 |
18.8 |
3.36 |
26.7 |
| CV% |
7.4 |
5.8 |
7.3 |
7.9 |
Recovery
The recovery of human MIP-3 beta spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
112 |
106-117 |
| EDTA Plasma (n=2) |
99 |
91-107 |
| Heparin Plasma (n=2) |
98 |
93-102 |
| Serum (n=2) |
98 |
94-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human MIP-3 beta were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data
Human CCL19/MIP-3 beta Quantikine QuicKit ELISA Standard Curve
Product Datasheets
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Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL19/MIP-3 beta
Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).
Entrez Gene IDs:
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names:
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19
