Human CCL2/JE/MCP-1 Antibody Summary
Gln24-Thr99
Accession # P13500
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) at 25 µg/mL for overnight at 4° C temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Chemotaxis Induced by CCL2/MCP‑1 and Neutral-ization by Human CCL2/ MCP‑1 Antibody. Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND50 is typically 1.5-4.5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported. Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells.
Product Datasheets
Citations for Human CCL2/JE/MCP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism
Authors: Collin de l'Hortet A, Takeishi K, Guzman-Lepe J et al.
Cell Metab.
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Multivariate analysis of traumatic brain injury: development of an assessment score.
Authors: Buonora JE, Yarnell AM, Lazarus RC et al.
Front Neurol
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Tumor-associated tissue eosinophilia promotes angiogenesis and metastasis in head and neck squamous cell carcinoma
Authors: Tsung-Lun Lee, Tien-Hua Chen, Ying-Ju Kuo, Hsin-Yi Lan, Muh-Hwa Yang, Pen-Yuan Chu
Neoplasia
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Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
Arch. Biochem. Biophys., 2019-12-24;0(0):108238.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
HIV‐1 Tat promotes astrocytic release of CCL2 through MMP/PAR‐1 signaling
Authors: P. Lorenzo Bozzelli, Tao Yin, Valeria Avdoshina, Italo Mocchetti, Katherine E. Conant, Kathleen A. Maguire‐Zeiss
Glia
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Characterization of Innate Immunity in an Extended Whole Blood Model of Human Islet Allotransplantation
Authors: Maria Hårdstedt, Susanne Lindblom, Alex Karlsson-Parra, Bo Nilsson, Olle Korsgren
Cell Transplantation
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Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
Autophagy, 2013-12-04;10(2):257-68.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells.
Authors: Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C
J Immunol, 2013-04-10;190(10):5321-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
Diabetes, 2011-05-01;60(5):1512-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Authors: Ouwehand K, Oosterhoff D, Breetveld M
J. Invest. Dermatol., 2010-11-11;131(2):418-25.
Species: Human
Sample Types: Whole Tissue
Applications: Neutralization -
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
Int. J. Cancer, 2009-09-15;125(6):1276-84.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Interleukin-18 predicts atherosclerosis progression in SIV-infected and uninfected rhesus monkeys (Macaca mulatta) on a high-fat/high-cholesterol diet.
Authors: Yearley JH, Xia D, Pearson CB, Carville A, Shannon RP, Mansfield KG
Lab. Invest., 2009-04-20;89(6):657-67.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis Rheum., 2008-11-01;58(11):3510-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
J. Neurosci., 2008-03-26;28(13):3277-90.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
J. Exp. Med., 2004-05-03;199(9):1213-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Alterations in expression of monocyte chemotactic protein-1 in the simian immunodeficiency virus model of disseminated Mycobacterium avium complex.
Authors: Hendricks EE, Lin KC, Boisvert K, Pauley D, Mansfield KG
J. Infect. Dis., 2004-04-13;189(9):1714-20.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
Authors: Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
Gene Ther., 2004-01-01;11(2):126-32.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-Fr -
Epitope Mapping of Mono- and Polyclonal Antibodies by Screening Phage-displayed Random Peptide Libraries
Authors: P Molek, T Bratkovi?
Acta Chim Slov, 2016-12-01;63(4):914-919.
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MCP-1 targeting: Shutting off an engine for tumor development
Authors: L Wang, J Lan, J Tang, N Luo
Oncology Letters, 2021-11-19;23(1):26.
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Interleukin-17 induces angiogenesis in�vitro via CXCL8 and CCL2 in retinal pigment epithelium
Authors: Y Chen, M Zhong, G Yuan, H Peng
Mol Med Rep, 2018-01-18;0(0):.
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After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC).
A: Paired with biotinylated anti-human CCL2 antibody (MAB279) as a capture reagent. Calibration curve with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)
B: Paired with biotinylated anti-porcine CCL2 antibody (Abcam, Cat# ab193793). Calibration curve with recombinant swine CCL2 (ImmunoChemistry Technologies, Cat# 6374) is shown (dynamic range 6-25,000 pg/ml).
