Home / CCL22/MDC / Human CCL22/MDC Biotinylated Antibody

Human CCL22/MDC Biotinylated Antibody

Catalog #: BAF336
7 Citations Datasheet / COA / SDS
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BAF336
Product Details
Citations (7)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL22/MDC Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL22/MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant mouse TARC, recombinant human (rh) TARC, and rh6Ckine is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
Gly25-Gln93
Accession # O00626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL22/MDC (Catalog # 336-MD)

Human CCL22/MDC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL22/MDC Antibody (Catalog # MAB336)

Standard: Recombinant Human CCL22/MDC Protein (Catalog # 336-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL22/MDC

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

References
  1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
  3. Pal, R. et al. (1997) Science 278:5338.
Entrez Gene IDs
6367 (Human); 20299 (Mouse)
Alternate Names
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

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Citations for Human CCL22/MDC Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation
    Authors: Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén
    Pediatric Research
  2. Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
    Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
    Cancers, 2021-03-16;13(6):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Detection
  3. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007-10-01;179(7):4840-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  5. CCR4 blockade does not inhibit allergic airways inflammation.
    Authors: Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I
    J. Leukoc. Biol., 2003-07-15;74(4):558-63.
    Species: Guinea Pig
    Sample Types: BALF
    Applications: ELISA Development
  6. Placental immune response to apple allergen in allergic mothers
    Authors: Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al.
    Journal of Reproductive Immunology
  7. A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization
    Authors: T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm
    Clinical & Experimental Allergy

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