Human CCL23/Ck beta 8-1 Antibody

Catalog #: MAB508 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB508
MAB508-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL23/Ck beta 8-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL23/Ck beta 8‑1 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with human CCL23/CK beta 8 isoform is observed.

Source

Monoclonal Mouse IgG₁ Clone # 103952

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL23/CK beta 8-1
Met46-Asn137
Accession # AAD10846

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL23/Ck beta 8‑1 aa 46-137 (Catalog # 508-CK)

Human CCL23/Ck beta 8-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL23/Ck beta 8‑1 Biotinylated Antibody (Catalog # BAF508)

Standard: Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein (Catalog # 508-CK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL23/Ck beta 8-1

CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu47‑Gly63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46‑132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22‑132).

References

Youn, B-S. et al. (1998) Blood 91:3118.
Macphee, C.H. et al. (1998) J. Immunol. 161:6273.

Entrez Gene IDs

6368 (Human)

Alternate Names

C6 beta-chemokine; CCL23/Ck beta 8-1; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; Ckb81; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23