Human CCL23/Ck beta 8-1 Antibody

Catalog #: AF508 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF508
AF508-SP

Chemotaxis Induced by CCL23 and Neutralization by Human CCL23 Antibody.

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Human CCL23/Ck beta 8-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects CCL23/Ck beta 8‑1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL23/Ck beta 8‑1
Met46-Asn137
Accession # AAD10846

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CCL23/Ck beta 8‑1 aa 46-137 (Catalog # 508-CK)

Neutralization

Measured by its ability to neutralize CCL23-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 0.15-0.75 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL23/MPIF‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL23 and Neutralization by Human CCL23 Antibody. Recombinant Human CCL23/MPIF-1 (Catalog # 371-MP) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF-1 (0.04 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CCL23/Ck beta 8-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF508). The ND₅₀ is typically 0.15-0.75 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL23/Ck beta 8-1

CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu 47‑Gly 63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46‑132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22‑132).

References

Youn, B-S. et al. (1998) Blood 91:3118.
Macphee, C.H. et al. (1998) J. Immunol. 161:6273.

Entrez Gene IDs

6368 (Human)

Alternate Names

C6 beta-chemokine; CCL23/Ck beta 8-1; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; Ckb81; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23