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Human CCL25/TECK Antibody

Catalog #: MAB334
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB334-SP
MAB334-100
MAB334-500
Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody.
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Human CCL25/TECK Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 21, 22, or recombinant rat CCL20.  is observed.
Source
Monoclonal Mouse IgG2B Clone # 52513
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL25/TECK
Gln24-Leu150
Accession # AAB69981
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 1.5 µg/mL Recombinant Human CCL25/TECK.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody. View Larger

Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody. Recombinant Human CCL25/TECK (Catalog # 334-TK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL25/TECK (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL25/ TECK Monoclonal Antibody (Catalog # MAB334). The ND50 is typically 3-12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor

References
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
Entrez Gene IDs
6370 (Human); 20300 (Mouse)
Alternate Names
C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine

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Citation for Human CCL25/TECK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003-12-04;103(7):2806-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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