Home / CCL26/Eotaxin-3 / Human CCL26/Eotaxin-3 Quantikine ELISA Kit

Human CCL26/Eotaxin-3 ELISA Kit - Quantikine

Catalog #: DCC260B
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DCC260B
Control Products Available
QC276: Quantikine Immunoassay Control Set 1034 for Human Eotaxin-3
Human Eotaxin-3 Simple Plex Assay Linearity
4 Images
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Human CCL26/Eotaxin-3 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.215 pg/mL
Assay Range
3.1 - 200 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Plasma)
Specificity
Natural and recombinant human Eotaxin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human CCL26/Eotaxin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Eotaxin-3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed, recombinant human Eotaxin-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 43.6 86 131 44.4 91.7 139
Standard Deviation 0.57 1.06 1.77 3.66 7.79 11.6
CV% 1.3 1.2 1.4 8.2 8.5 8.3

Recovery

The recovery of human Eotaxin-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 92-119
Cell Lysis Buffer (n=1) 97 94-99
EDTA Plasma (n=5) 94 84-105
Heparin Plasma (n=5) 95 86-109
Serum (n=5) 93 81-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Eotaxin-3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Eotaxin-3 Simple Plex Assay Linearity
Human Eotaxin-3 ELISA Linearity

Scientific Data

N/A View Larger

Human Eotaxin-3 ELISA Standard Curve

N/A Human Eotaxin-3 ELISA Standard Curve View Larger

Human Eotaxin-3 ELISA Standard Curve

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Proton pump inhibitors decrease eotaxin-3 expression in the proximal esophagus of children with esophageal eosinophilia.
    Authors: Park, Jason Y, Zhang, Xi, Nguyen, Nathalie, Souza, Rhonda F, Spechler, Stuart J, Cheng, Edaire
    PLoS ONE, 2014-07-02;9(7):e101391.
    Species: Human
    Sample Types: Whole Cells
  2. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2013-10-28;164(2):286-95.
    Species: Human
    Sample Types: Plasma
  3. Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
    Authors: Provost V, Langlois A, Chouinard F, Rola-Pleszczynski M, Chakir J, Flamand N, Laviolette M
    PLoS ONE, 2012-08-31;7(8):e43544.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid.
    Authors: Kagami S, Kai H, Kakinuma T
    J. Invest. Dermatol., 2011-09-01;132(1):249-51.
    Species: Human
    Sample Types: Serum
  5. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Fujita H
    J. Invest. Dermatol., 2010-05-27;130(9):2304-11.
    Species: Human
    Sample Types: Serum
  6. Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells.
    Authors: Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS
    Cytokine, 2008-11-26;44(3):342-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
    Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
    J. Allergy Clin. Immunol., 2007-12-20;121(2):348-54.
    Species: Human
    Sample Types: Serum
  8. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  9. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2007-03-09;36(5):237-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Regulatory mechanisms of galectin-9 and eotaxin-3 synthesis in epidermal keratinocytes: possible involvement of galectin-9 in dermal eosinophilia of Th1-polarized skin inflammation.
    Authors: Igawa K, Satoh T, Hirashima M, Yokozeki H
    Allergy, 2006-12-01;61(12):1385-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Anti-IL-5 (mepolizumab) therapy for eosinophilic esophagitis.
    Authors: Stein ML, Collins MH, Villanueva JM, Kushner JP, Putnam PE, Buckmeier BK, Filipovich AH, Assa&amp;apos;ad AH, Rothenberg ME
    J. Allergy Clin. Immunol., 2006-11-07;118(6):1312-9.
    Species: Human
    Sample Types: Plasma
  12. Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
    Authors: Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD
    Eur. J. Immunol., 2006-10-01;36(10):2700-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Markers of eosinophilic inflammation and risk prediction in patients with coronary artery disease.
    Authors: Falcone C, Minoretti P, D&amp;apos;Angelo A, Buzzi MP, Coen E, Emanuele E, Aldeghi A, Olivieri V, Geroldi D
    Eur. J. Clin. Invest., 2006-04-01;36(4):211-7.
    Species: Human
    Sample Types: Plasma
  14. Eotaxin-3 and a uniquely conserved gene-expression profile in eosinophilic esophagitis.
    Authors: Assa&amp;apos;ad AH
    J. Clin. Invest., 2006-02-01;116(2):536-47.
    Species: Human
    Sample Types: Plasma
  15. Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions.
    Authors: Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW
    Chest, 2005-06-01;127(6):2094-100.
    Species: Human
    Sample Types: Pleural Effusion

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human CCL26/Eotaxin-3 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human CCL26/Eotaxin-3 Quantikine ELISA Kit and earn rewards!

Have you used Human CCL26/Eotaxin-3 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review