Human CCL27/CTACK Antibody

Name: Human CCL27/CTACK Antibody
Brand: R&D Systems
SKU: MAB3761
Rating: 4.3 (4 reviews)

Catalog #: MAB3761
4 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3761
MAB3761-SP

All CCL27/CTACK Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (4)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL27/CTACK Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL27/CTACK in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CCL27, recombinant human (rh) CCL25, or rhCCL19 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 124302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CCL27/CTACK
Phe25-Gly112
Accession # NP_006655

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of human skin

Human CCL27/CTACK Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL27/CTACK Biotinylated Antibody (Catalog # BAF376)

Standard: Recombinant Human CCL27/CTACK Protein (Catalog # 376-CT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).

References

Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
Gortz, A. et al. (2002) J. Immunol. 169:1387.
Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
Homey, B. et al. (2000) J. Immunol. 164:3465.
Homey, B. et al. (2002) Nat. Med. 8:157.
Inokuma, D. et al. (2006) Stem Cells 24:2810.
Jarmin, D. et al. (2000) J. Immunol. 164:3460.
Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
Soler, D. et al. (2003) Blood 101:1677.
Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Entrez Gene IDs

10850 (Human); 20301 (Mouse); 362505 (Rat)

Alternate Names

ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL27/CTACK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Phenotypic characterization of leukocytes in prenatal human dermis.
J. Invest. Dermatol., 2012-06-21;132(11):2581-92.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Lymphatic precollectors contain a novel, specialized subpopulation of podoplanin low, CCL27-expressing lymphatic endothelial cells.
Authors: Wick N, Haluza D, Gurnhofer E, Raab I, Kasimir MT, Prinz M, Steiner CW, Reinisch C, Howorka A, Giovanoli P, Buchsbaum S, Krieger S, Tschachler E, Petzelbauer P, Kerjaschki D
Am. J. Pathol., 2008-09-04;173(4):1202-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr, IHC-P
Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
Arthritis Rheum., 2005-05-01;52(5):1504-16.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
TARC augments TNF-alpha-induced CTACK production in keratinocytes.
Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
Exp. Dermatol., 2004-09-01;13(9):551-7.
Species: Human
Sample Types: Cell Culture Supernates