Human CCL27/CTACK Biotinylated Antibody Summary
Phe25-Gly112
Accession # Q9Y4X3
Applications
Human CCL27/CTACK Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL27/CTACK
CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11% - 35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7-9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).
- Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
- Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
- Gortz, A. et al. (2002) J. Immunol. 169:1387.
- Morales, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96:14470.
- Homey, B. et al. (2000) J. Immunol. 164:3465.
- Homey, B. et al. (2002) Nat. Med. 8:157.
- Inokuma, D. et al. (2006) Stem Cells 24:2810.
- Jarmin, D. et al. (2000) J. Immunol. 164:3460.
- Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
- Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
- Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
- Soler, D. et al. (2003) Blood 101:1677.
- Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
- Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
- Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.
Product Datasheets
Citation for Human CCL27/CTACK Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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TARC augments TNF-alpha-induced CTACK production in keratinocytes.
Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
Exp. Dermatol., 2004-09-01;13(9):551-7.
Species: Human
Sample Types: Cell Culture Supernates
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