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Human CCL28 Antibody

Catalog #: AF717
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF717
AF717-SP
Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL28 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CCL28 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL28 (Catalog # 717-VC)
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 40-100 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. View Larger

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717). The ND50 is typically 40-100 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

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Citations for Human CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma
    Authors: Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al.
    Laboratory Investigation
  2. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.
    Authors: Danilova, E, Skrindo, I, Gran, E, Hales, B J, Smith, W A, Jahnsen, J, Johansen, F E, Jahnsen, F L, Baekkevold, E S
    Mucosal Immunol, 2014-06-11;8(1):107-14.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
    Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
    J. Immunol., 2003-02-01;170(3):1452-61.
    Species: Human
    Sample Types: Milk
    Applications: Western Blot

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