Human CCL3/CCL4 PE-conjugated Antibody

Name: Human CCL3/CCL4 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC2701P

Catalog #: IC2701P
4 Citations Datasheet / COA / SDS

Discontinued Product

IC2701P has been discontinued.

View all CCL3/CCL4 products.

Detection of CCL3/CCL4 in Human Blood Monocytes by Flow Cytometry.

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All CCL3/CCL4 Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL3/CCL4 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL3/MIP-1 alpha in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed. Does not cross-react with rhCCL1, 2, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 5, 6, 7, CCL9/10MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.

Source

Monoclonal Mouse IgG_2B Clone # 93342

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CCL3/CCL4 antibody in Human Blood Monocytes antibody by Flow Cytometry.

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Detection of CCL3/CCL4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL3/CCL4 PE-conjugated Monoclonal Antibody (Catalog # IC2701P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL3/CCL4

The Macrophage Inflammatory Proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptors for MIP-1 alpha have been identified as CCR1 and CCR5.

References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Long Name

CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive

Alternate Names

C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3; CCL3/CCL4

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human CCL3/CCL4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Human Decidual CD1a+ Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96
Authors: T Gulic, G Laskarin, L Glavan, T Grubi? Kez, H Haller, D Rukavina
International Journal of Molecular Sciences, 2023-01-23;24(3):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.
Authors: Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I
J Immunol, 2015-08-14;195(6):2861-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
Species: Human
Sample Types: Whole Cells
Applications: Chip Cytometry
Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
Blood, 2008-01-18;111(7):3579-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry