Home / CCL4/MIP-1 beta / Human CCL4/MIP-1 beta MAb (Clone 24006)

Human CCL4/MIP-1 beta MAb (Clone 24006)

Catalog #: MAB271
26 Citations 5 Reviews Datasheet / COA / SDS
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MAB271-100
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Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody.
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Citations (26)
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Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL4/MIP-1 beta MAb (Clone 24006) Summary

Species Reactivity
Human
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human
(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 24006
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Human CCL4/MIP-1 beta Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL4/MIP‑1 beta Biotinylated Antibody (Catalog # BAF271)

Standard: Recombinant Human CCL4/MIP-1 beta Protein (Catalog # 271-BME)

Neutralization
Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP‑1 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody. View Larger

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody. Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically 0.3-1.2 µg/mL.

Intracellular Staining by Flow Cytometry Detection of CCL4/MIP-1 beta antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL4/MIP-1 beta

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Entrez Gene IDs
6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Citations for Human CCL4/MIP-1 beta MAb (Clone 24006)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
    Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
    Frontiers in Immunology
  2. A Novel Highly Potent Therapeutic Antibody Neutralizes Multiple Human Chemokines and Mimics Viral Immune Modulation
    Authors: Michelle L. Scalley-Kim, Bruce W. Hess, Ryan L. Kelly, Anne-Rachel F. Krostag, Kurt H. Lustig, John S. Marken et al.
    PLoS ONE
  3. High levels of CCL2 or CCL4 in the tumor microenvironment predict unfavorable survival in lung adenocarcinoma
    Authors: Lei Li, Yong-Dong Liu, Yu-Ting Zhan, Ying-Hui Zhu, Yan Li, Dan Xie et al.
    Thoracic Cancer
  4. Modulation of the CCR5 Receptor/Ligand Axis by Seminal Plasma and the Utility of In Vitro versus In Vivo Models
    Authors: Jennifer A. Juno, Kathleen M. Wragg, Anne B. Kristensen, Wen Shi Lee, Kevin J. Selva, Renee M. van der Sluis et al.
    Journal of Virology
  5. Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease
    Authors: Marcus Buggert, Son Nguyen, Laura M. McLane, Maria Steblyanko, Nadia Anikeeva, Dominic Paquin-Proulx et al.
    PLOS Pathogens
  6. LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis
    Authors: Wang, J;Chai, Q;Lei, Z;Wang, Y;He, J;Ge, P;Lu, Z;Qiang, L;Zhao, D;Yu, S;Qiu, C;Zhong, Y;Li, BX;Zhang, L;Pang, Y;Gao, GF;Liu, CH;
    EMBO molecular medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTOF-reported
  7. A keratinocyte-adipocyte signaling loop is reprogrammed by loss of BTG3 to augment skin carcinogenesis
    Authors: Cheng, YC;Acedera, JD;Li, YJ;Shieh, SY;
    Cell death and differentiation
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. A map of transcriptional heterogeneity and regulatory variation in human microglia
    Authors: AMH Young, N Kumasaka, F Calvert, TR Hammond, A Knights, N Panousis, JS Park, J Schwartzen, J Liu, K Kundu, M Segel, NA Murphy, CE McMurran, H Bulstrode, J Correia, KP Budohoski, A Joannides, MR Guilfoyle, R Trivedi, R Kirollos, R Morris, MR Garnett, I Timofeev, I Jalloh, K Holland, R Mannion, R Mair, C Watts, SJ Price, PJ Kirkpatric, T Santarius, E Mountjoy, M Ghoussaini, N Soranzo, OA Bayraktar, B Stevens, PJ Hutchinson, RJM Franklin, DJ Gaffney
    Nature Genetics, 2021-06-03;53(6):861-868.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia
    Authors: A van Hooij, EM Tjon Kon F, M Batista da, R Carvalho B, AC Cunha Mess, AR Gobbo, T Lema, K Bobosha, J Li, X Weng, CG Salgado, JS Spencer, PLAM Corstjens, A Geluk
    Sci Rep, 2018-12-18;8(1):17920.
    Species: Human
    Sample Types: Whole Cells
    Applications: Lateral Flow
  10. Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
    Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
    PLoS Pathog., 2018-02-23;14(2):e1006888.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer.
    Authors: Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H
    Clin Cancer Res, 2015-04-08;21(10):2268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISpot Development
  12. HIV-1-induced impairment of dendritic cell cross talk with gammadelta T lymphocytes.
    Authors: Cardone M, Ikeda K, Varano B, Gessani S, Conti L
    J Virol, 2015-02-11;89(9):4798-808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009-07-28;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  15. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
    Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
    Clin. Immunol., 2008-04-22;128(1):57-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  16. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  17. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008-02-01;214(3):328-36.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  18. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  20. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  21. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006-07-01;54(7):2096-108.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  22. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid, Cell Culture Supernates
    Applications: ELISA Development, Neutralization
  23. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
    Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
    J. Virol., 2006-01-01;80(1):236-45.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  24. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  25. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)
    Authors: Conor N. Gruber, Roosheel S. Patel, Rebecca Trachtman, Lauren Lepow, Fatima Amanat, Florian Krammer et al.
    Cell
  26. Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety
    Authors: Nicholas A. Vitanza, Ashley L. Wilson, Wenjun Huang, Kristy Seidel, Christopher Brown, Joshua A. Gustafson et al.
    Cancer Discovery

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Reviews for Human CCL4/MIP-1 beta MAb (Clone 24006)

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Human CCL4/MIP-1 beta MAb (Clone 24006)
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Worked as a capture IgG for the NHP ELISA

ELISA Human CCL4/MIP-1 beta MAb (Clone 24006) MAB271
Human CCL4/MIP-1 beta Antibody
By Anonymous on 08/20/2021
Application: WB Sample Tested: Serum Species: Human
Western Blot Human CCL4/MIP-1 beta Antibody MAB271
Human CCL4/MIP-1 beta Antibody
By Anonymous on 08/19/2019
Application: ELISA Sample Tested: Serum Species: Human
ELISA Human CCL4/MIP-1 beta Antibody MAB271
Human CCL4/MIP-1 beta Antibody
By Michelle Chen on 04/26/2017
Application: B/N Sample Tested: Peripheral blood neutrophils Species: Human

Neutrophil derived CCL4 was neutralized in co-culture with A375 tumor cells. This effect on the extravasation of A375 MA2 cells was assessed.

Block/Neutralize Human CCL4/MIP-1 beta Antibody MAB271
Human CCL4/MIP‑1 beta Antibody
By Anonymous on 04/25/2016
Application: Affinity Purification Sample Tested: HUVEC human umbilical vein endothelial cells Species: Dengue virus