Human CCL7/MCP-3/MARC Antibody Summary
Gln34-Leu109
Accession # Q7Z7Q8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/MCP‑3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL7/MCP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-282-NA). The ND50 is typically 10-30 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL7/MCP-3/MARC
MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.
CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Product Datasheets
Citation for Human CCL7/MCP-3/MARC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Evidence for chemokine synergy during neutrophil migration in ARDS
Thorax, 2016-08-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Used as a capture antibody in our NHP ELISA
The polyclonal antibody AF282 was used as both capture and detection in a sandwich ELISA for MCP-3 in human samples. It worked great!
