Human CCR1 Antibody

Catalog #: MAB145R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB145R-100
MAB145R-SP

Detection of CCR1 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR1 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 53504R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145R) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR1

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Alternate Names

C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R