Human CCR1 Biotinylated Antibody

Name: Human CCR1 Biotinylated Antibody
Brand: R&D Systems
SKU: FAB145B

Catalog #: FAB145B
1 Citation Datasheet / COA / SDS

Clone 53504 was used by HLDA to establish CD designation

Discontinued Product

FAB145B has been discontinued.

View all CCR1 products.

Detection of CCR1 in Human PBMCs by Flow Cytometry.

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Citations (1)

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Human CCR1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry. No cross‑reactivity with CCR5 transfectants is observed.

Source

Monoclonal Mouse IgG_2B Clone # 53504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human CCR1 Biotinylated Monoclonal Antibody (Catalog # FAB145B) or (B) Mouse IgG_2BBiotinylated Isotype Control (Catalog # IC0041B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCR1

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Alternate Names

C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CCR1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004-12-01;173(11):6592-602.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry