Human CCR10 Antibody

Catalog #: MAB3478R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3478R-100
MAB3478R-SP

Detection of CCR10 in HEK293 Human Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR10 transfectants but not the parental cell line in Flow Cytometry.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 314305R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR10 in HEK293 Human Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CCR10 and eGFP was stained with (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478R) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Alternate Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2