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Human CCR10 APC-conjugated Antibody

Catalog #: FAB3478A
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3478A-025
FAB3478A-100
Detection of CCR10 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR10 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 314305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR10 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Rat Anti-Human CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB3478A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR10 antibody in L1.2 Mouse Cell Line Transfected with Human CCR10 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR10 in L1.2 Mouse Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry. L1.2 mouse pro-B cell line transfected with human CCR10 and eGFP was stained with either (A) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A) or (B) Rat Anti-Human CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB3478A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27 and CCL28. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression is correlated with the metastatic behavior of melanomas.

Entrez Gene IDs
2826 (Human); 12777 (Mouse); 363682 (Rat)
Alternate Names
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

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Citations for Human CCR10 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Time-resolved systems immunology reveals a late juncture linked to fatal COVID-19
    Authors: Can Liu, Andrew J. Martins, William W. Lau, Nicholas Rachmaninoff, Jinguo Chen, Luisa Imberti et al.
    Cell
  2. Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries
    Authors: SY Kim-Wang, AG Holt, AM McGowan, ST Danyluk, AP Goode, BC Lau, AP Toth, JR Wittstein, LE DeFrate, JS Yi, AL McNulty
    Arthritis Research & Therapy, 2021-11-04;23(1):280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS
    Authors: T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker
    Cancer Res., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.
    Authors: Danilova, E, Skrindo, I, Gran, E, Hales, B J, Smith, W A, Jahnsen, J, Johansen, F E, Jahnsen, F L, Baekkevold, E S
    Mucosal Immunol, 2014-06-11;8(1):107-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica.
    Authors: Chihara, Norio, Aranami, Toshimas, Oki, Shinji, Matsuoka, Takako, Nakamura, Masakazu, Kishida, Hitaru, Yokoyama, Kazumasa, Kuroiwa, Yoshiyuk, Hattori, Nobutaka, Okamoto, Tomoko, Murata, Miho, Toda, Tatsushi, Miyake, Sachiko, Yamamura, Takashi
    PLoS ONE, 2013-12-10;8(12):e83036.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010-09-09;116(24):5181-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells.
    Authors: Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M
    Infect. Immun., 2008-04-21;76(7):3304-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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