Human CCR10 PerCP-conjugated Antibody

Name: Human CCR10 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB3478C

Catalog #: FAB3478C Datasheet / COA / SDS

Discontinued Product

FAB3478C has been discontinued.

View all CCR10 products.

Detection of CCR10 in L1.2 Mouse Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry.

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Human CCR10 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 314305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR10 in L1.2 Mouse Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry. L1.2 mouse pro-B cell line transfected with human CCR10 and eGFP was stained with either (A) Rat Anti-Human CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3478C) or (B) Rat IgG_2APerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR10 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Rat Anti-Human CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3478C) or (B) Rat IgG_2APerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Alternate Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2