Human CCR11 APC-conjugated Antibody

Name: Human CCR11 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB6899A

Catalog #: FAB6899A Datasheet / COA / SDS

Discontinued Product

FAB6899A has been discontinued.

View all CCR11 products.

Detection of CCR11 in NS0 Mouse Cell Line Transfected with Human CCR11 and eGFP by Flow Cytometry.

2 Images

All CCR11 Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR11 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR11 in direct ELISAs.

Source

Monoclonal Mouse IgM Clone # 674144

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CCR11
Accession # Q9NPB9

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CCR11 in NS0 Mouse Cell Line Transfected with Human CCR11 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCR11 and eGFP was stained with Mouse Anti-Human CCR11 APC-conjugated Monoclonal Antibody (Catalog # FAB6899A). Quadrant markers were set based on control antibody staining (Catalog # IC015A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of CCR11 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human CCR11 APC-conjugated Monoclonal Antibody (Catalog # FAB6899A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR11

CCR11, also known as CCRL1 and CCX-CKR, is a 45 kDa 7-transmembrane segment protein that is widely expressed on stromal cells. It is a silent receptor that does not induce intracellular calcium signaling, but instead serves as a chemokine scavenger receptor. CCR11 regulates dendritic cell and thymic precursor migration by mediating the clearance of the homeostatic chemokines CCL19, 21, and 25. It is upregulated on some breast cancer cells and on bronchial epithelial cells in pulmonary sarcoidosis. Human CCR11 shares 85% amino acid sequence identity with mouse and rat CCR11.

Entrez Gene IDs

51554 (Human); 252837 (Mouse); 685243 (Rat)

Alternate Names

ACKR4; CC chemokine receptor-like 1; C-C CKR-11; CCBP2CCX CKR; CC-CKR-11; CCR11; CCR11CCR10; CCRL1; CCX-CKR; chemokine (C-C motif) receptor-like 1; orphan seven-transmembrane receptor, chemokine related; PPR1; PPR1cc motif, receptor-like protein 1; VSHK1; VSHK1CKR-11