Human CCR5 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB182P-025
FAB182P-100
Detection of CCR5 in NS0 Mouse Cell Line Transfected with Human CCR5 by Flow Cytometry.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)

Human CCR5 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR5.
Source
Monoclonal Mouse IgG2B Clone # 45531
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR5 antibody in NS0 Mouse Cell Line Transfected with Human CCR5 antibody by Flow Cytometry. View Larger

Detection of CCR5 in NS0 Mouse Cell Line Transfected with Human CCR5 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCR5 was stained with Mouse Anti-Human CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB182P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

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Citations for Human CCR5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Spermine and spermidine bind CXCR4 and inhibit CXCR4- but not CCR5-tropic HIV-1 infection
    Authors: Harms, M;Smith, N;Han, M;Gro beta, R;von Maltitz, P;Stürzel, C;Ruiz-Blanco, YB;Almeida-Hernández, Y;Rodriguez-Alfonso, A;Cathelin, D;Caspar, B;Tahar, B;Sayettat, S;Bekaddour, N;Vanshylla, K;Kleipass, F;Wiese, S;Ständker, L;Klein, F;Lagane, B;Boonen, A;Schols, D;Benichou, S;Sanchez-Garcia, E;Herbeuval, JP;Münch, J;
    Science advances
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  2. Rapid identification of highly potent human anti-GPCR antagonist monoclonal antibodies
    Authors: Martin J. Scott, Amanda Jowett, Martin Orecchia, Peter Ertl, Larissa Ouro-Gnao, Julia Ticehurst et al.
    mAbs
  3. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012-06-01;42(6):1547-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011-09-01;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Role of the atypical chemoattractant receptor CRAM in regulating CCL19 induced CCR7 responses in B-cell chronic lymphocytic leukemia
    Authors: Julie Catusse, Marion Leick, Mareike Groch, David J Clark, Maike V Buchner, Katja Zirlik et al.
    Molecular Cancer
  6. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009-11-01;183(9):5494-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006-05-01;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CCR5 PE-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human CCR5 PE-conjugated Antibody
By Anonymous on 12/11/2017
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

Human CCR5 PE-conjugated Antibody
By Anonymous on 06/28/2017
Application: Flow Sample Tested: U937 human histiocytic lymphoma cell line Species: Human