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Human CCRL2/CRAM-A/B Antibody

Catalog #: MAB23501
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB23501
MAB23501-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCRL2/CRAM-A/B Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.
Source
Monoclonal Mouse IgG2B Clone # 152254
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CRAM-A
Met1-Val356 (Val180Met)
Accession # NP_001124382
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HL‑60 human acute promyelocytic leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCRL2/CRAM-A/B

CCRL2, also known as CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the inclusion of an additional 12 amino acids on CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, and T cells (1, 2).

References
  1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
  2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
Long Name
CC Chemokine Receptor-like 2
Entrez Gene IDs
9034 (Human)
Alternate Names
ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor

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Citations for Human CCRL2/CRAM-A/B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
    Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
    PLoS ONE, 2023-01-20;18(1):e0280590.
    Species: Human
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Flow Cytometry, Neutralization, Surface Plasmon Resonance Micr
  2. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
    Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
    Immunology, 2009-12-02;129(4):536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human B cells express the orphan chemokine receptor CRAM-A/B in a maturation-stage-dependent and CCL5-modulated manner.
    Authors: Hartmann TN, Leick M, Ewers S, Diefenbacher A, Schraufstatter I, Honczarenko M, Burger M
    Immunology, 2008-04-04;125(2):252-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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