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Human CCRL2/CRAM-A/B APC-conjugated Antibody

Catalog #: FAB23501A
2 Citations Datasheet / COA / SDS
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FAB23501A
Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCRL2/CRAM-A/B APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.
Source
Monoclonal Mouse IgG2B Clone # 152254
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CRAM-A
Met1-Val356 (Val180Met)
Accession # NP_001124382
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCRL2/CRAM-A/B antibody in PBMCs antibody by Flow Cytometry. View Larger

Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry. Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCRL2/CRAM‑A/B in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (IC0041A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCRL2/CRAM-A/B

CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.

References
  1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
  2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
  3. Hartmann, T.N. et al. (2008) Immunology 125:252.
  4. Leick, M. et al. (2010) Immunology 129:536.
Long Name
CC Chemokine Receptor-like 2
Entrez Gene IDs
9034 (Human)
Alternate Names
ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor

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Citations for Human CCRL2/CRAM-A/B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mesenchymal stromal cell-secreted chemerin is a novel immunomodulatory molecule driving the migration of ChemR23-expressing cells
    Authors: Paola Vinci
    Cytotherapy, 2016-12-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration
    Authors: Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A. Schneider, Medhanie A. Mulaw et al.
    Oncotarget

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