Human CCRL2/CRAM-A/B PE-conjugated Antibody

Name: Human CCRL2/CRAM-A/B PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB23501P

Catalog #: FAB23501P Datasheet / COA / SDS

Discontinued Product

FAB23501P has been discontinued.

View all CCRL2/CRAM-A/B products.

Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry.

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All CCRL2/CRAM-A/B Antibodies

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Human CCRL2/CRAM-A/B PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.

Source

Monoclonal Mouse IgG_2B Clone # 152254

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CRAM-A
Met1-Val356 (Val180Met)
Accession # NP_001124382

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry. Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B PE-conjugated Monoclonal Antibody (Catalog # FAB23501P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCRL2/CRAM-A/B

CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.

References

Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
Hartmann, T.N. et al. (2008) Immunology 125:252.
Leick, M. et al. (2010) Immunology 129:536.

Long Name

CC Chemokine Receptor-like 2

Entrez Gene IDs

9034 (Human)

Alternate Names

ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor