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Human CD-M6PR Lumenal Domain Antibody

Catalog #: AF5320
1 Citation Datasheet / COA / SDS
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AF5320
AF5320-SP
Detection of Human CD-M6PR Lumenal Domain by Western Blot.
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Human CD-M6PR Lumenal Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD-M6PR Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant mouse CD-M6PR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD-M6PR Lumenal Domain
Thr27-His185
Accession # P20645
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD-M6PR Lumenal Domain antibody by Western Blot. View Larger

Detection of Human CD-M6PR Lumenal Domain by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD-M6PR Lumenal Domain at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD-M6PR

The CD-M6PR (cation-dependent mannose 6-phosphate receptor; also CD-MPR) is a 44‑46 kDa member of the P-type lectin family of proteins. It is ubiquitously expressed, found internally, and transports newly formed mannose 6-phosphate bearing acid hydrolases from the secretory pathway to acidified lysosomes. Mature human CD-M6PR is a type I transmembrane glycoprotein that is 251 amino acids (aa) in length. It functions as a homodimer, and contains a 159 aa luminal region (aa 27‑185) plus a 67 aa cytoplasmic domain. The aa residues Gln66, Arg111, Glu133 and Tyr143 recognize carbohydrate; Asp103 interacts with divalent cation (Mn2+), increasing ligand affinity and receptor oligomerization. Over aa 27‑184, human CD-M6PR shares 90% aa identity with mouse CD-M6PR.

Long Name
Cation Dependent Mannose-6-Phosphate Receptor 46 kDa
Entrez Gene IDs
4074 (Human); 17113 (Mouse); 312689 (Rat)
Alternate Names
000 Man6PR; 46 kDa mannose 6-phosphate receptor; 46-kDa mannose 6-phosphate receptor; cation-dependent mannose-6-phosphate receptor; CD Man-6-P receptor; CDM6PR; CD-M6PR; CD-MPR; FLJ32994; M6PR; mannose-6-phosphate receptor (cation dependent); MPR 46; MPR46; MPRD; Mr 46; SMPR

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Citation for Human CD-M6PR Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
    Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
    Acta Neuropathol Commun, 2019-01-31;7(1):13.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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