Human CD14 Antibody
Human CD14 Antibody Summary
Thr20-Cys352
Accession # P08571
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD14 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 5 µg/mL Human CD14 Monoclonal Antibody (Catalog # MAB3831) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human CD14 (Catalog # 383-CD) (5 ng) was included. A specific band for CD14 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD14
CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) precursor protein with a 19 aa signal peptide and a C‑terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
- Wright, S.D. et al. (1990) Science 249:1431.
- Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
- Beutler, B. (2000) Current Opinion in Immunology 12:20.
- Stelter, F. (2000) Chem. Immunol. 74:25.
Product Datasheets
Citations for Human CD14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells.
Authors: Izban M, Nowicki B, Nowicki S
PLoS ONE, 2012-11-12;7(11):e49318.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
Clin. Exp. Immunol., 2009-03-09;156(2):285-93.
Species: Human
Sample Types: Saliva
Applications: Immunodepletion, Western Blot -
Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome.
Authors: Srinivasan M, Kodumudi KN, Zunt SL
Clin. Immunol., 2007-10-03;126(1):31-7.
Species: Human
Sample Types: Saliva
Applications: Western Blot
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Completed this experiment three times. Cells were either lysed in RIPA buffer or EasyPep lysis buffer. Positive control worked every time, but antibody didn't work for actual protein lysate. I used MAB3832 under non-reducing conditions and it worked right away at 1:100.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
