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Human CD14 Biotinylated Antibody

Catalog #: BAF383
14 Citations 2 Reviews Datasheet / COA / SDS
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BAF383
CD14 in Human Lymph Node.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD14 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD14 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse CD14 and Lipopolysaccharide is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD14 (Catalog # 383-CD)
Immunohistochemistry
5-15 µg/mL
Immersion fixed THP‑1 human acute monocytic leukemia cell line (Positive), MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) and U937 human histiocytic lymphoma cell line (Positive).

Human CD14 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CD14 Antibody (Catalog # MAB3833)

Capture Reagent: Human CD14 Antibody (Catalog # MAB3833R)

Standard: Recombinant Human CD14 Protein (Catalog # 383-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CD14 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

CD14 in Human Lymph Node. CD14 was detected in immersion fixed paraffin-embedded sections of human lymph node using 10 µg/mL Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-AEC Cell & Tissue Staining Kit (red; CTS020) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry View Larger

Detection of CD14 in THP‑1 (Positive) and MOLT‑4 (Negative). CD14 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (Positive) and absent in MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) using Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry View Larger

Detection of CD14 in U937(Positive) and MOLT‑4 (Negative). CD14 was detected in immersion fixed U937 human histiocytic lymphoma cell line (Positive) and absent in MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) using Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Citations for Human CD14 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
    Authors: SA Aschenbroi, ER Lafontaine, MC Lopez, HV Baker, RJ Hogan
    BMC Med Genomics, 2019-09-06;12(1):127.
  2. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
    Authors: Komori M, Blake A, Greenwood M et al.
    Ann Neurol
  3. Decreased serpin C1 in extracellular vesicles predicts response to methotrexate treatment in patients with pulmonary sarcoidosis
    Authors: Kraaijvanger, R;Janssen Bonás, M;Grutters, JC;Paspali, I;Veltkamp, M;de Kleijn, DPV;van Moorsel, CHM;
    Respiratory research
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  4. Immunologic biomarkers, morbidity and mortality among HIV patients hospitalised in a Tertiary Care Hospital in the Brazilian Amazon
    Authors: WM Gama, CHM Frank, TVR Almeida, DS Dos Santos, YO Chaves, DF da Silva, PP Orlandi, FR Pereira, GF Magalhães, BJ Baptista, VL de Oliveir, AA da Silva B, MF Santana, RL Gonçalves, AG da Costa, MC Dos Santos, LC de Lima Fe, MVG Lacerda, PA Nogueira
    BMC infectious diseases, 2021-08-26;21(1):876.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis
    Authors: B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh
    PLoS ONE, 2020-06-03;15(6):e0234038.
    Species: Human
    Sample Types: Whole Tissues
    Applications: IHC
  6. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis
    Authors: X Hou, G Chen, W Bracamonte, HS Choi, NL Diny, J Sung, D Hughes, T Won, MK Wood, MV Talor, DJ Hackam, K Klingel, G Davogustto, H Taegtmeyer, I Coppens, JG Barin, D ?iháková
    Cell Rep, 2019-07-02;28(1):172-189.e7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
    Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
    PLoS ONE, 2016-01-28;11(1):e0148073.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  8. Pharmacological Inhibition of Protein Kinase G1 Enhances Bone Formation by Human Skeletal Stem Cells Through Activation of RhoA-Akt Signaling.
    Authors: Jafari A, Siersbaek M, Chen L, Qanie D, Zaher W, Abdallah B, Kassem M
    Stem Cells, 2015-04-23;33(7):2219-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  9. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  10. Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract.
    Authors: Blais DR, Harrold J, Altosaar I
    Pediatr. Res., 2006-03-01;59(3):371-6.
    Species: Human
    Sample Types: Milk
    Applications: Western Blot
  11. Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation.
    Authors: Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaisse JM
    Cancer Res., 2005-11-01;65(21):9943-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Affinity Purification
  12. Effect of Early-Stage Human Breast Carcinoma on Monocyte Programming
    Authors: Marina Patysheva, Irina Larionova, Marina Stakheyeva, Evgeniya Grigoryeva, Pavel Iamshchikov, Natalia Tarabanovskaya et al.
    Frontiers in Oncology
  13. CX3CR1-fractalkine axis drives kinetic changes of monocytes in fibrotic interstitial lung diseases
    Authors: Greiffo FR, Viteri-Alvarez V, Frankenberger M et al.
    Eur. Respir. J.
  14. PFKFB3 overexpression in monocytes of patients with colon but not rectal cancer programs pro-tumor macrophages and is indicative for higher risk of tumor relapse
    Authors: Larionova I, Patysheva M, Iamshchikov P et al.
    Frontiers in immunology

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Human CD14 Biotinylated Antibody
By Anonymous on 10/14/2021
Application: ELISA Sample Tested: Whole blood Species: Human

The 1:1000 dilution secondary anti-human CD14 Biotinylated Antibody was added.

ELISA Human CD14 Biotinylated Antibody BAF383
Human CD14 Biotinylated Antibody
By Anonymous on 07/27/2016
Application: Flow Sample Tested: Peripheral blood cells Species: Human