Human CD163 Antibody

Catalog #: MAB11580 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11580-100
MAB11580-SP

Detection of CD163 in A375 Human Cell Line.

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Human CD163 Antibody Summary

Species Reactivity

Human

Specificity

Detects rhCD163 in Direct ELISA

Source

Monoclonal Mouse IgG_2B Clone # 1084111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0-derived human CD163
Gly41-Ser1045
Accession # Q86VB7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

see below

Immunocytochemistry

3-25 µg/mL

Immersion fixed A375 human melanoma cell line and HDLM-2 human Hodgkin's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of CD163 in A375 Human Cell Line. CD163 was detected in immersion fixed A375 human melanoma cell line and not detected in HDLM‑2 human Hodgkin’s lymphoma cell line using Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB11580) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry

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Detection of CD163 in PBMC by Flow Cytometry PBMC were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB11580) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References

Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
Sulahian, T.H. et al. (2000) Cytokine 12:1312.
Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
Kristiansen, M. et al. (2001) Nature 409:198.
Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
Moller, H.J. et al. (2002) Blood 99:378.
Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
Schaer, D.J. et al. (2006) Blood 107:373.
Bover, L.C. et al. (2007) J. Immunol. 178:8183.
Fabriek, B.O. et al. (2007) Blood 109:5223.

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Alternate Names

CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163