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Human CD163 Antibody

Catalog #: MAB1607
13 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1607-SP
MAB1607-100
MAB1607-500
Detection of CD163 in PBMC by Flow Cytometry
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD163 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD163 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 215927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD163
Gly46-Ser1050
Accession # Q86VB7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD163 (Catalog # 1607-CD) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
Human PBMC monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD163 in PBMC by Flow Cytometry PBMC were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB1607) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References
  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

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Citations for Human CD163 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Potential Role of CXCL10 in Monitoring Response to Treatment in Leprosy Patients
    Authors: Helen Ferreira, Mayara Abud Mendes, Mayara Garcia de Mattos Barbosa, Eliane Barbosa de Oliveira, Anna Maria Sales, Milton Ozório Moraes et al.
    Frontiers in Immunology
  2. Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling
    Authors: C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh
    PLoS ONE, 2021-04-22;16(4):e0250107.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Murlentamab, a Low Fucosylated Anti-M�llerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation
    Authors: M Prat, M Salon, T Allain, O Dubreuil, G Noël, L Preisser, B Jean, L Cassard, F Lemée, I Tabah-Fish, B Pipy, P Jeannin, JF Prost, JM Barret, A Coste
    Cancers, 2021-04-13;13(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Macrophage Polarization in Leprosy–HIV Co-infected Patients
    Authors: Tatiana Pereira da Silva, Tamiris Lameira Bittencourt, Ariane Leite de Oliveira, Rhana Berto da Silva Prata, Vinicius Menezes, Helen Ferreira et al.
    Frontiers in Immunology
  5. Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth
    Authors: C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik
    Cancer Res., 2018-09-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2
    Authors: N McGovern, A Shin, G Low, D Low, K Duan, LJ Yao, R Msallam, I Low, NB Shadan, HR Sumatoh, E Soon, J Lum, E Mok, S Hubert, P See, EH Kunxiang, YH Lee, B Janela, M Choolani, CNZ Mattar, Y Fan, TKH Lim, DK Hong, KK Tan, JKC Tam, C Schuster, A Elbe-Bürge, XN Wang, V Bigley, M Collin, M Haniffa, A Schlitzer, M Poidinger, S Albani, A Larbi, EW Newell, JKY Chan, F Ginhoux
    Nature, 2017-06-14;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  7. Jagged1 Instructs Macrophage Differentiation in Leprosy
    PLoS Pathog, 2016-08-17;12(8):e1005808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A CCL24-dependent pathway augments eosinophilic airway inflammation in house dust mite-challenged Cd163(-/-) mice
    Authors: Cuilian Dai, Xianglan Yao, Elizabeth M. Gordon, Amisha Barochia, Rosemarie A. Cuento, Maryann Kaler et al.
    Mucosal Immunology
  9. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
    Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
    Cancer Res, 2012-09-12;72(22):5733-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
    Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
    Clin. Cancer Res., 2011-04-13;17(14):4642-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages.
    Authors: Thelen T, Hao Y, Medeiros AI
    J. Immunol., 2010-09-01;185(7):4328-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
    Authors: Ochiel DO, Ghosh M, Fahey JV
    J. Leukoc. Biol., 2010-04-12;88(3):435-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
    Authors: Choi KD, Vodyanik MA, Slukvin II
    J. Clin. Invest., 2009-08-10;119(9):2818-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CD163 Antibody
By Anonymous on 11/12/2021
Application: WB Sample Tested: Serum Species: Human
Western Blot Human CD163 Antibody MAB1607
Human CD163 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human CD163 Antibody MAB1607
Human CD163 Antibody
By Carly Garrison on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human CD163 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human