Human CD163 APC-conjugated Antibody Summary
Gly46-Ser1050
Accession # Q86VB7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD163 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD163 APC-conjugated Monoclonal Antibody (Catalog # FAB1607A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD163
CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).
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- Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
- Kristiansen, M. et al. (2001) Nature 409:198.
- Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
- Moller, H.J. et al. (2002) Blood 99:378.
- Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
- Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
- Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
- Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
- Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
- Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
- Schaer, D.J. et al. (2006) Blood 107:373.
- Bover, L.C. et al. (2007) J. Immunol. 178:8183.
- Fabriek, B.O. et al. (2007) Blood 109:5223.
Product Datasheets
Citations for Human CD163 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The effect of caveolin-1 knockdown on interleukin-1 beta -induced chemokine (C-C motif) ligand 2 expression in synovial fluid-derived fibroblast-like synoviocytes from patients with rheumatoid arthritis
Authors: Dorota Trzybulska, Anna Olewicz-Gawlik, Jan Sikora, Magdalena Frydrychowicz, Agata Kolecka-Bednarczyk, Mariusz Kaczmarek et al.
Advances in Clinical and Experimental Medicine
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Complement inhibitor factor H expressed by breast cancer cells differentiates CD14+ human monocytes into immunosuppressive macrophages
Authors: Karolina I. Smolag, Christine M. Mueni, Karin Leandersson, Karin Jirström, Catharina Hagerling, Matthias Mörgelin et al.
OncoImmunology
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Hypoxia-induced complement component 3 promotes aggressive tumor growth in the glioblastoma microenvironment
Authors: Rosberg, R;Smolag, KI;Sjölund, J;Johansson, E;Bergelin, C;Wahldén, J;Pantazopoulou, V;Ceberg, C;Pietras, K;Blom, AM;Pietras, A;
JCI insight
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Exosomal ?v?6 integrin is required for monocyte M2 polarization in prostate cancer
Authors: H Lu, N Bowler, LA Harshyne, D Craig Hoop, SR Krishn, S Kurtoglu, C Fedele, Q Liu, HY Tang, AV Kossenkov, WK Kelly, K Wang, RB Kean, PH Weinreb, L Yu, A Dutta, P Fortina, A Ertel, M Stanczak, F Forsberg, DI Gabrilovic, DW Speicher, DC Altieri, LR Languino
Matrix Biol., 2018-03-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages.
Authors: Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C
J Thorac Oncol, 2016-07-12;11(10):1765-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Modulation of Decidual Macrophage Polarization by Macrophage Colony-Stimulating Factor Derived from First-Trimester Decidual Cells: Implication in Preeclampsia.
Authors: Li M, Piao L, Chen C, Wu X, Yeh C, Masch R, Chang C, Huang S
Am J Pathol, 2016-03-10;186(5):1258-66.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A phase 1/2 study combining gemcitabine, Pegintron and p53 SLP vaccine in patients with platinum-resistant ovarian cancer.
Authors: Dijkgraaf E, Santegoets S, Reyners A, Goedemans R, Nijman H, van Poelgeest M, van Erkel A, Smit V, Daemen T, van der Hoeven J, Melief C, Welters M, Kroep J, van der Burg S
Oncotarget, 2015-10-13;6(31):32228-43.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Ipilimumab-dependent cell-mediated cytotoxicity of regulatory T cells ex vivo by nonclassical monocytes in melanoma patients.
Authors: Romano E, Kusio-Kobialka M, Foukas P, Baumgaertner P, Meyer C, Ballabeni P, Michielin O, Weide B, Romero P, Speiser D
Proc Natl Acad Sci U S A, 2015-04-27;112(19):6140-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor, Walther, Michael
J Immunol, 2012-10-24;189(11):5336-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Authors: Johnson TR, McLellan JS, Graham BS
J. Virol., 2011-11-16;86(3):1339-47.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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